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human cell lines nih 3t3 mouse embryonic fibroblasts  (ATCC)


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    ATCC human cell lines nih 3t3 mouse embryonic fibroblasts
    Human Cell Lines Nih 3t3 Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 13189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nih+3t3+mouse+fibroblast+cells/NIH%2F3T3/pm42229414-756-2-9
    Average 99 stars, based on 13189 article reviews
    human cell lines nih 3t3 mouse embryonic fibroblasts - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Organ-Specific Metabolite Profiling of Mahonia aquifolium (Pursh) Nutt. Extracts by GC-FID/MS and UHPLC-HRMS/MS with Bioactivity Assessment
    Article Snippet: .. NIH/3T3 mouse fibroblast cells (ATCC, CRL-1658) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin until they reached 70–80% confluence. .. Similarly, human monocyte THP-1 cells (ATCC, TIB-202) were maintained in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% FBS, 1% penicillin–streptomycin, and 0.05 mM 2-mercaptoethanol (Gibco, 2198–023) under 5% CO 2 at 37 °C until 70–80% confluence was achieved.

    Article Title: Plasma‐Processed Bioprinted Chitosan Hydrogels: Enhancing Physicochemical and Antibacterial Properties for Wound Healing
    Article Snippet: .. NIH/3T3 mouse fibroblast cells (ATCC) were cultured in a 50/50 mixture of DMEM high glucose and Ham's F12 with L‐glutamine, supplemented with 10% FBS and 1% PS. ..

    Modification:

    Article Title: Organ-Specific Metabolite Profiling of Mahonia aquifolium (Pursh) Nutt. Extracts by GC-FID/MS and UHPLC-HRMS/MS with Bioactivity Assessment
    Article Snippet: .. NIH/3T3 mouse fibroblast cells (ATCC, CRL-1658) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin until they reached 70–80% confluence. .. Similarly, human monocyte THP-1 cells (ATCC, TIB-202) were maintained in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% FBS, 1% penicillin–streptomycin, and 0.05 mM 2-mercaptoethanol (Gibco, 2198–023) under 5% CO 2 at 37 °C until 70–80% confluence was achieved.

    Polymer:

    Article Title: Agro-Industrial Waste Valorization for Sustainable PHBV Production from Sugarcane Bagasse Using Bacillus sp. HLI02.
    Article Snippet: .. The cytotoxicity of the polymer was checked on NIH/3T3 mouse fibroblast cells (ATCC CRL 1658) through viability (MTT) assay. .. Cells were grown in Dulbecco’s Modified Eagle Medium containing 1.5 g/L glucose (DMEM-Low Glucose, HiMedia, India), added with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and 1% antibiotic (100 U/mL of penicillin and 100 μg/mL streptomycin; from Hi-Media, India) in the incubation was maintained at 37 ◦C with 5% CO2 and 95% RHS.

    Article Title: Agro-Industrial Waste Valorization for Sustainable PHBV Production from Sugarcane Bagasse Using Bacillus sp. HLI02
    Article Snippet: .. The cytotoxicity of the polymer was checked on NIH/3T3 mouse fibroblast cells (ATCC CRL 1658) through viability (MTT) assay. .. Cells were grown in Dulbecco’s Modified Eagle Medium containing 1.5 g/L glucose (DMEM-Low Glucose, HiMedia, India), added with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and 1% antibiotic (100 U/mL of penicillin and 100 μg/mL streptomycin; from Hi-Media, India) in the incubation was maintained at 37 °C with 5% CO 2 and 95% RHS.

    MTT Assay:

    Article Title: Agro-Industrial Waste Valorization for Sustainable PHBV Production from Sugarcane Bagasse Using Bacillus sp. HLI02.
    Article Snippet: .. The cytotoxicity of the polymer was checked on NIH/3T3 mouse fibroblast cells (ATCC CRL 1658) through viability (MTT) assay. .. Cells were grown in Dulbecco’s Modified Eagle Medium containing 1.5 g/L glucose (DMEM-Low Glucose, HiMedia, India), added with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and 1% antibiotic (100 U/mL of penicillin and 100 μg/mL streptomycin; from Hi-Media, India) in the incubation was maintained at 37 ◦C with 5% CO2 and 95% RHS.

    Article Title: Agro-Industrial Waste Valorization for Sustainable PHBV Production from Sugarcane Bagasse Using Bacillus sp. HLI02
    Article Snippet: .. The cytotoxicity of the polymer was checked on NIH/3T3 mouse fibroblast cells (ATCC CRL 1658) through viability (MTT) assay. .. Cells were grown in Dulbecco’s Modified Eagle Medium containing 1.5 g/L glucose (DMEM-Low Glucose, HiMedia, India), added with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and 1% antibiotic (100 U/mL of penicillin and 100 μg/mL streptomycin; from Hi-Media, India) in the incubation was maintained at 37 °C with 5% CO 2 and 95% RHS.



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    Effect of 10 Gy X‐ray on viability of <t>NIH‐3T3,</t> B16.F10, MDA‐MB‐231, and HepG2 cells. Blue: untreated controls; Pink: irradiated cells. Cell counts were conducted at 24, 48, and 72 h post‐treatment. Statistical analysis was performed using two‐way ANOVA with Tukey's post hoc test: p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***), p < 0.0001 (****), n = 3. Note: Y ‐axes are scaled independently for each cell line to account for differences in baseline growth, allowing clearer visualization of treatment effects. Tumor cell counts and representative images can be found in Appendix Table and Figure .
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    Image Search Results


    Effect of 10 Gy X‐ray on viability of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells. Blue: untreated controls; Pink: irradiated cells. Cell counts were conducted at 24, 48, and 72 h post‐treatment. Statistical analysis was performed using two‐way ANOVA with Tukey's post hoc test: p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***), p < 0.0001 (****), n = 3. Note: Y ‐axes are scaled independently for each cell line to account for differences in baseline growth, allowing clearer visualization of treatment effects. Tumor cell counts and representative images can be found in Appendix Table and Figure .

    Journal: MicrobiologyOpen

    Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

    doi: 10.1002/mbo3.70270

    Figure Lengend Snippet: Effect of 10 Gy X‐ray on viability of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells. Blue: untreated controls; Pink: irradiated cells. Cell counts were conducted at 24, 48, and 72 h post‐treatment. Statistical analysis was performed using two‐way ANOVA with Tukey's post hoc test: p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***), p < 0.0001 (****), n = 3. Note: Y ‐axes are scaled independently for each cell line to account for differences in baseline growth, allowing clearer visualization of treatment effects. Tumor cell counts and representative images can be found in Appendix Table and Figure .

    Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

    Techniques: Irradiation

    Images of untreated NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells and cells exposed to X‐ray. Scalebar: 300 µm.

    Journal: MicrobiologyOpen

    Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

    doi: 10.1002/mbo3.70270

    Figure Lengend Snippet: Images of untreated NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells and cells exposed to X‐ray. Scalebar: 300 µm.

    Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

    Techniques:

    Viability of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells after treatment with IMP‐1700 (5 µM), ciprofloxacin (CPX) (15 µM), their combination, or DMSO, with and without 10 Gy X‐ray. Cell counts were measured at 24, 48, and 72 h post‐treatment. Statistical significance assessed via two‐way ANOVA and Tukey's post hoc test: p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***), p < 0.0001 (****), n = 3. Significant differences are only shown compared to untreated or 10 Gy. Tumor cell count values and images are available in Appendix Table and Figure , , , .

    Journal: MicrobiologyOpen

    Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

    doi: 10.1002/mbo3.70270

    Figure Lengend Snippet: Viability of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells after treatment with IMP‐1700 (5 µM), ciprofloxacin (CPX) (15 µM), their combination, or DMSO, with and without 10 Gy X‐ray. Cell counts were measured at 24, 48, and 72 h post‐treatment. Statistical significance assessed via two‐way ANOVA and Tukey's post hoc test: p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***), p < 0.0001 (****), n = 3. Significant differences are only shown compared to untreated or 10 Gy. Tumor cell count values and images are available in Appendix Table and Figure , , , .

    Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

    Techniques: Cell Characterization

    Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with IMP‐1700, with and without X‐ray. Scalebar: 300 µm.

    Journal: MicrobiologyOpen

    Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

    doi: 10.1002/mbo3.70270

    Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with IMP‐1700, with and without X‐ray. Scalebar: 300 µm.

    Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

    Techniques:

    Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with ciprofloxacin (CPX), with and without X‐ray. Scalebar: 300 µm.

    Journal: MicrobiologyOpen

    Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

    doi: 10.1002/mbo3.70270

    Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with ciprofloxacin (CPX), with and without X‐ray. Scalebar: 300 µm.

    Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

    Techniques:

    Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with IMP‐1700 and ciprofloxacin (CPX), with and without X‐ray. Scalebar: 300 µm.

    Journal: MicrobiologyOpen

    Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

    doi: 10.1002/mbo3.70270

    Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with IMP‐1700 and ciprofloxacin (CPX), with and without X‐ray. Scalebar: 300 µm.

    Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

    Techniques:

    Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with DMSO, with and without X‐ray. Scalebar: 300 µm.

    Journal: MicrobiologyOpen

    Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

    doi: 10.1002/mbo3.70270

    Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with DMSO, with and without X‐ray. Scalebar: 300 µm.

    Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

    Techniques:

    The cytocompatibility and antibacterial activity evaluation of the dECM@QCS and exo@dECM@QCS composite hydrogels. ( A ) Live cell staining was used to investigate the fibroblast proliferation (Scale = 100 μm). ( B ) Cell scratch assay was used to investigate the fibroblast migration (Scale = 100 μm). ( C ) Quantitative analysis results of the cell scratch experiment. ( D ) The CCK-8 experiment was used to detect the activity of fibroblasts. ( E ) The antibacterial properties of different materials were detected by plate counting method. “ns” indicates no statistical difference, * p < 0.05, and ** p < 0.01.

    Journal: Gels

    Article Title: Tendon dECM Composited with Chitosan with Loading Skin Precursor Stem Cell Exosome for Enhanced Diabetic Wound Healing

    doi: 10.3390/gels12050361

    Figure Lengend Snippet: The cytocompatibility and antibacterial activity evaluation of the dECM@QCS and exo@dECM@QCS composite hydrogels. ( A ) Live cell staining was used to investigate the fibroblast proliferation (Scale = 100 μm). ( B ) Cell scratch assay was used to investigate the fibroblast migration (Scale = 100 μm). ( C ) Quantitative analysis results of the cell scratch experiment. ( D ) The CCK-8 experiment was used to detect the activity of fibroblasts. ( E ) The antibacterial properties of different materials were detected by plate counting method. “ns” indicates no statistical difference, * p < 0.05, and ** p < 0.01.

    Article Snippet: The cytocompatibility of dECM@QCS and exo@dECM@QCS composite hydrogels was investigated by culturing with mouse embryonic fibroblast cells (NIH 3T3, ATCC, CRL-1658TM, Manassas, VA, USA) using fluorescent images and Cell Counting Kit-8 (CCK-8) assay [ ].

    Techniques: Activity Assay, Staining, Wound Healing Assay, Migration, CCK-8 Assay